Journal: Advanced Science

Article Title: An Integrated Optogenetic and Bioelectronic Platform for Regulating Cardiomyocyte Function

doi: 10.1002/advs.202402236

Figure Lengend Snippet: Expression of CM markers after illumination. Immunofluorescence images of NT and bPAC‐expressing CMs exhibiting cytoskeletal α ‐actinin, Cx‐43 gap junctions, and mCherry. Nuclear DNA staining by DAPI is also shown. Prior to preparation for imaging, the cells were illuminated at 27 µW mm −2 for 30 min. Scale bar: 20 µm.

Article Snippet: Samples were washed three times (5 min each time) with PBS under light rotation and blocked with 5% BSA in PBS for 1 h. Antibodies against Connexin‐43 (rabbit; Abcam, cat. no. ab11370, Cambridge, MA), α ‐actinin (mouse, Sigma, cat. no. A7811, St. Louis, MO), or mCherry (goat, OriGene, cat. no. TA150126, Rockville, MD) were added overnight at 4 °C in 1% BSA in PBS.

Techniques: Expressing, Immunofluorescence, Staining, Imaging

Journal: Scientific Reports

Article Title: Integrins mediate placental extracellular vesicle trafficking to lung and liver in vivo

doi: 10.1038/s41598-021-82752-w

Figure Lengend Snippet: Key resources. Information includes species and strains of animals used in this study, software, kits, antibodies, and reagents.

Article Snippet: Antibody , Anti-EGFP (goat polyclonal) , Origene , Origene:TA150095 , (1:2000).

Techniques: Software, Imaging, Labeling, Isolation, Recombinant, Plasmid Preparation